By Ashok Agarwal, Sajal Gupta, Rakesh Sharma
This cutting-edge laboratory handbook comprises 20 medical protocols used day-by-day for the research of the infertile male, provided with effortless to appreciate, step by step technique. The protocols are prepared from regimen to complex laboratory tactics universal to medical perform, together with computer-assisted semen research, sperm coaching for IUI by way of density gradient and swim-up, sperm cryopreservation, and sperm DNA fragmentation attempt via TUNEL technique, between others. The method in every one protocol follows most sensible perform instructions made clearer through professionally hand-drawn illustrations protecting many of the very important steps and gear. The authors, hailing from the world-renowned Andrology heart at Cleveland medical institution, have over 50 years of mixed first-hand event in coping with very busy diagnostic and examine amenities in male infertility and andrology. The e-book could be an quintessential source for millions of laboratory technologists, clinicians and reproductive pros (andrologists, embryologist, etc.) engaged within the prognosis and administration of infertile males round the world.
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Additional info for Andrological Evaluation of Male Infertility: A Laboratory Guide
Hum Reprod. 1995;10: 2096–106. 22. De Jonge C. Commentary: forging a partnership between total quality management and the andrology laboratory. J Androl. 2000; 21:203–5. 2 Quality Management in Andrology Laboratory 23. Bielanski A, Bergeron H, Lau PCK, Devenish J. Microbial contamination of embryos and semen during long term banking in liquid nitrogen. Cryobiol. 2003;46:146–52. 24. Bielanski A. Experimental microbial contamination and disinfection of dry (vapour) shipper Dewars designed for short-term storage and transportation of cryopreserved germplasm and other biological specimens.
Sanitation of each preparation area before and after use. Before bringing a specimen into the work area, lay out and label all of the materials to be used for that specimen. All contact materials should be labeled with the patient’s name and a secondary identiﬁer unless it is a disposable labware that will not leave the hand of the worker from the time it contacts the semen until it is discarded into a biohazardous waste container. Once the specimen, in its sealed container, is placed in its preparation area, no other specimens or contact materials may enter the designated area unless they are sterile material still in their packaging or sealed containers labeled with the patient’s name.
43. Knuth UA, Neuwinger J, Nieschlag E. Bias to routine semen analysis by uncontrolled changes in laboratory environment – detection by long-term sampling of monthly means for quality control. Int J Androl. 1989;12:375–83. 44. Walker RH. Pilot surveys for proﬁciency testing of semen analysis. Comparison of dry-ice vs. liquid nitrogen shipments. Arch Pathol Lab Med. 1992;116:423–4. 45. Johnson JE, Blackhurst DW, Boone WR. Can Westgard quality control rules determine the suitability of frozen sperm pellets as a control material for computer assisted semen analyzers?