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Extra info for 3D Histology Evaluation of Dermatologic Surgery
7d). From this specimen, the bottom is cut away (Fig. 7d, e) and the tissue left above can be taken for tumor diagnostics. The bottom (base) and the entire margin of the excised sample are now transformed from its 3D outside into a 2D specimen with the outside facing down (Fig. 7e). Now, embedding, horizontal slicing, and staining can be performed, and after staining, tumor-positive areas can be detected (Fig. 7f). Two-Dimensional Scheme, Comparing Oblique and Vertical Incisions and Flattening of 3D Margins Here the differences between the two above described methods of incision are compared in a 2D scheme side by side.
7a, b) so that the entire margin of 22 Chapter 2. 8 Two-dimensional schemes, comparing oblique (a) and vertical (b) incision and flattening of 3D margins the specimen is represented in one strip starting left from the 12 o’clock suture marker (Fig. 7b, c). This strip can then be laid flat with the outside of the surface facing down (Fig. 7c). Following the removal of the margins, the tumor center with its base was left behind (Fig. 7d). From this specimen, the bottom is cut away (Fig. 7d, e) and the tissue left above can be taken for tumor diagnostics.
To investigate the sections, the pathologist first analyses the last and deepest (nearest to the tumor) section. This section normally covers the entire outside of the incision and should be representative and of best quality. Therefore, tumor outgrowths are seen here at best and easiest. If tumor outgrowths are found in this section, three interpretation possibilities have to be distinguished. Possibilities for Evaluation of Complete or Incomplete Excision In the first example (Fig. 11, 1) shown here, a tumor outgrowth is found between 6 and 9 o’clock in the last and deepest section (nearest to the tumor) (Fig.